anti paxl y779 (R&D Systems)
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Anti Paxl Y779, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+axl+y779/Human+Phospho-Axl+(Y779)+Antibody/pmc12595455__pnas%2E2502778122%2Esapp-2-25-27
Average 93 stars, based on 55 article reviews
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Blocking Assay:Article Title: Targeting the Axl and mTOR Pathway Synergizes Immunotherapy and Chemotherapy to Butylidenephthalide in a Recurrent GBM Article Snippet: Protein lysates of primary culture cells isolated from the GBM were resolved on sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGEs) and electro-transferred to polyvinylidene fluoride (PVDF) membrane (Merck Millipore) in a wet blot format. .. After protein transfer, the membranes were blocked with blocking buffer (5% non-fat dry milk powder, dissolved in 0.1% PBS-Tween 20) and incubated with primary antibodies against MGMT (2739S, Cell Signaling, Beverly, MA, USA), EGFR (GTX628887, GeneTex, Hsinchu, Taiwan), EGFR (phosphor Tyr1068) (GTX132810, GeneTex, Hsinchu, Taiwan), Axl (H-124) (sc-20741, Santa Cruz Biotechnology, Dallas, TX, USA), Incubation:Article Title: Targeting the Axl and mTOR Pathway Synergizes Immunotherapy and Chemotherapy to Butylidenephthalide in a Recurrent GBM Article Snippet: Protein lysates of primary culture cells isolated from the GBM were resolved on sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGEs) and electro-transferred to polyvinylidene fluoride (PVDF) membrane (Merck Millipore) in a wet blot format. .. After protein transfer, the membranes were blocked with blocking buffer (5% non-fat dry milk powder, dissolved in 0.1% PBS-Tween 20) and incubated with primary antibodies against MGMT (2739S, Cell Signaling, Beverly, MA, USA), EGFR (GTX628887, GeneTex, Hsinchu, Taiwan), EGFR (phosphor Tyr1068) (GTX132810, GeneTex, Hsinchu, Taiwan), Axl (H-124) (sc-20741, Santa Cruz Biotechnology, Dallas, TX, USA), Western Blot:Article Title: Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice. Article Snippet: Fluorescent labeling of CD68 was achieved using secondary FITC coupled anti-rat IgG STAR 80F (Serotec). .. Fifty lg of protein from snap frozen liver were used for Western blotting as previously described [16] using antibodies against PCNA (Sigma), Article Title: Targeting Tyro3, Axl, and MerTK Receptor Tyrosine Kinases Significantly Sensitizes Triple-Negative Breast Cancer to CDK4/6 Inhibition Article Snippet: Proteins were separated using SDS-PAGE (Gel = Bolt 4–12% Bis-Tris, Invitrogen (Waltham, MA, USA), NW04120BOX), transferred onto the PVDF membranes (Immobilon-FL, Millipore Sigma, (St. Louis, MO, USA), IPFL00005), and subsequently blocked (Intercept Blocking Buffer, Li-Cor, Lincoln, NE, USA, 92760001) for 1 h prior to the addition of primary antibodies. .. The following antibodies were used for immunoblotting: phospho-Met (Tyr1234/1235) (CST, 3077), Met (D1C2) (CST, 8198), Axl (C89E7) (CST, 8661), Article Title: SKI‐G‐801, an AXL kinase inhibitor, blocks metastasis through inducing anti‐tumor immune responses and potentiates anti‐PD‐1 therapy in mouse cancer models Article Snippet: Equivalent amounts of protein were separated using the NuPAGE 4–12% Bis–Tris Gel System (Invitrogen) and then transferred to polyvinylidene difluoride membranes. .. Membranes were probed with anti‐phospho‐AXL (Y702) (1:1000, CTS, #5724; Cell Signaling Technology, MA, USA), Article Title: Gilteritinib, a FLT3/AXL inhibitor, shows antileukemic activity in mouse models of FLT3 mutated acute myeloid leukemia Article Snippet: MV4–11-AXL cells, which exogenously express AXL, were also prepared at Astellas Pharma Inc. Ba/F3 cells expressing FLT3-ITD, FLT3-D835Y, FLT3-ITD-D835Y, FLT3-ITD-F691 L, or FLT3-ITD-F691I mutations (FLT3-ITD_Ba/F3, FLT3-D835Y_Ba/F3, FLT3-ITD-D835Y_Ba/F3, FLT3-ITD-F691L_Ba/F3, FLT3-ITD-F691I_Ba/F3) were established by Astellas Pharma Inc. .. The following antibodies were used for immunoblotting: FLT3 (Abcam, Cambridge, MA); phosphorylated pan-tyrosine (Merck Millipore, Billerica, MA); p44/42 MAPK (ERK1/2), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (197G2) (E10), AKT, phospho-AKT (Ser473) (193H12), and AXL (C44G1) (Cell Signaling Technology, Danvers, MA); STAT5 and phospho-STAT5 (Y694) (BD Biosciences, Franklin Lakes, NJ); Affinity Purification:Article Title: Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis Article Snippet: .. For the detection of phosphorylated Axl, an affinity-purified rabbit Stripping:Article Title: SKI‐G‐801, an AXL kinase inhibitor, blocks metastasis through inducing anti‐tumor immune responses and potentiates anti‐PD‐1 therapy in mouse cancer models Article Snippet: Equivalent amounts of protein were separated using the NuPAGE 4–12% Bis–Tris Gel System (Invitrogen) and then transferred to polyvinylidene difluoride membranes. .. Membranes were probed with anti‐phospho‐AXL (Y702) (1:1000, CTS, #5724; Cell Signaling Technology, MA, USA), |
